Publications and Preprints



Rowe JB, Taghon GJ, Kapolka NJ, Morgan WM, Isom DG. CRISPR-addressable yeast strains with applications in human G protein-coupled receptor profiling and synthetic biology. J Biol Chem. 2020;295(24):8262-8271. doi:10.1074/jbc.RA120.013066


Kapolka NJ, Taghon GJ, Rowe JB, Morgan WM, Enten JF, Isom DG. DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery. Proc Natl Acad Sci U S A. 2020;117(23):13117-13126. doi:10.1073/pnas.2000430117


Kapolka NJ, Taghon GJ, Rowe JB, Morgan WM, Enten JF, Isom DG. 2020. DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery. bioRxiv doi:



Lassuthova P, Rebelo AP, Ravenscroft G, Lamont PJ, Davis MR, Manganelli F, Feely SM, Bacon C, Brožková DŠ, Haberlova J, Mazanec R, Tao F, Saghira C, Abreu L, Courel S, Powell E, Buglo E, Bis DM, Baxter MF, Ong RW, Marns L, Lee YC, Bai Y, Isom DG, Barro-Soria R, Chung KW, Scherer SS, Larsson HP, Laing NG, Choi BO, Seeman P, Shy ME, Santoro L, Zuchner S. Mutations in ATP1A1 Cause Dominant Charcot-Marie-Tooth Type 2. Am J Hum Genet. 2018 Mar 1;102(3):505-514. doi: 10.1016/j.ajhg.2018.01.023. PubMed PMID: 29499166; PubMed Central PMCID: PMC5985288.


Isom DG, Page SC, Collins LB, Kapolka NJ, Taghon GJ, Dohlman HG. Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast. J Biol Chem. 2018 Feb 16;293(7):2318-2329. doi: 10.1074/jbc.RA117.000422. Epub 2017 Dec 28. PubMed PMID: 29284676; PubMed Central PMCID: PMC5818193.



Shellhammer JP, Morin-Kensicki E, Matson JP, Yin G, Isom DG, Campbell SL, Mohney RP, Dohlman HG. Amino acid metabolites that regulate G protein signaling during osmotic stress. PLoS Genet. 2017 May;13(5):e1006829. doi: 10.1371/journal.pgen.1006829. eCollection 2017 May. PubMed PMID: 28558063; PubMed Central PMCID: PMC5469498.



Isom DG, Sridharan V, Dohlman HG. Regulation of Ras Paralog Thermostability by Networks of Buried Ionizable Groups. Biochemistry. 2016 Jan 26;55(3):534-42. doi: 10.1021/acs.biochem.5b00901. Epub 2016 Jan 6. PubMed PMID: 26701741; PubMed Central PMCID: PMC5574170.



Isom DG, Dohlman HG. Buried ionizable networks are an ancient hallmark of G protein-coupled receptor activation. Proc Natl Acad Sci U S A. 2015 May 5;112(18):5702-7. doi: 10.1073/pnas.1417888112. Epub 2015 Apr 20. PubMed PMID: 25902551; PubMed Central PMCID: PMC4426463.



Baker R, Wilkerson EM, Sumita K, Isom DG, Sasaki AT, Dohlman HG, Campbell SL. Differences in the regulation of K-Ras and H-Ras isoforms by monoubiquitination. J Biol Chem. 2013 Dec 27;288(52):36856-62. doi: 10.1074/jbc.C113.525691. Epub 2013 Nov 18. PubMed PMID: 24247240; PubMed Central PMCID: PMC3873545.


Isom DG, Sridharan V, Baker R, Clement ST, Smalley DM, Dohlman HG. Protons as second messenger regulators of G protein signaling. Mol Cell. 2013 Aug 22;51(4):531-8. doi: 10.1016/j.molcel.2013.07.012. Epub 2013 Aug 15. PubMed PMID: 23954348; PubMed Central PMCID: PMC3770139.



Isom DG, Marguet PR, Oas TG, Hellinga HW. A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity. Proteins. 2011 Apr;79(4):1034-47. doi: 10.1002/prot.22932. Epub 2011 Jan 5. PubMed PMID: 21387407; PubMed Central PMCID: PMC3139720.


Isom DG, Castañeda CA, Cannon BR, García-Moreno B. Large shifts in pKa values of lysine residues buried inside a protein. Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5260-5. doi: 10.1073/pnas.1010750108. Epub 2011 Mar 9. PubMed PMID: 21389271; PubMed Central PMCID: PMC3069169.



Isom DG, Castañeda CA, Cannon BR, Velu PD, García-Moreno E B. Charges in the hydrophobic interior of proteins. Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16096-100. doi: 10.1073/pnas.1004213107. Epub 2010 Aug 26. PubMed PMID: 20798341; PubMed Central PMCID: PMC2941338.


Isom DG, Vardy E, Oas TG, Hellinga HW. Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4908-13. doi: 10.1073/pnas.0910421107. Epub 2010 Mar 1. PubMed PMID: 20194783; PubMed Central PMCID: PMC2841886.



Harms MJ, Castañeda CA, Schlessman JL, Sue GR, Isom DG, Cannon BR, García-Moreno E B. The pK(a) values of acidic and basic residues buried at the same internal location in a protein are governed by different factors. J Mol Biol. 2009 May 29;389(1):34-47. doi: 10.1016/j.jmb.2009.03.039. Epub 2009 Mar 24. PubMed PMID: 19324049; PubMed Central PMCID: PMC3373015.



Isom DG, Cannon BR, Castañeda CA, Robinson A, García-Moreno B. High tolerance for ionizable residues in the hydrophobic interior of proteins. Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17784-8. doi: 10.1073/pnas.0805113105. Epub 2008 Nov 12. PubMed PMID: 19004768; PubMed Central PMCID: PMC2584708.



Brun L, Isom DG, Velu P, García-Moreno B, Royer CA. Hydration of the folding transition state ensemble of a protein. Biochemistry. 2006 Mar 21;45(11):3473-80. doi: 10.1021/bi052638z. PubMed PMID: 16533028; PubMed Central PMCID: PMC4442614.



Gong H, Isom DG, Srinivasan R, Rose GD. Local secondary structure content predicts folding rates for simple, two-state proteins. J Mol Biol. 2003 Apr 11;327(5):1149-54. doi: 10.1016/s0022-2836(03)00211-0. PubMed PMID: 12662937.


isom lab